Is it possible to go
CopyMemory Dest, Source + 1, -1 ?

I dunno maybe if i did 1&.
vb6 has no support for unsigned integers. only unsigned bytes.
The first statement is more of just a security precaution incase if the uint ends up >= 32678. Which will bring back the signed integer value from a signed long, os i don't get an overflow error. Then vb will automatically convert that back to integer. Although i could probably just put exit sub, but its kinda there just incase.
For signed numbers as soon as you go past the limit it starts going backwards. A 3bit signed integer would go, 0 1 2 3 -4 -3 -2 -1. So to get -1 you would need 111 which is an unsigned value of 7 (the maximum possible) .That means to turn an integer > 32677 into an signed integer, you have to bitwise OR with FFFF on the left word to flip it.
You were correct on the second statement, but i will hopefully enlighten you with the first statement.
I will explain it in logical terms, because if i explained it in bitwise terms, only you would understand.

If the Last Bit of the Left-Most Bit = True, then the byte value must be >= 128 (or signed -128), but because it is the left-most bit, then the actual number must be > 32677, outside the range of a signed integer.
Bitshift 8 bits to the left using the the long of Hex100 (using the trailing & to declare it as long), making those 8 bits the most significant bits.
Lets say the value of the bytes were MSB=128 and LSB=255. This should give a Unsigned value of 33023.
That means for the first stage.
Attachment:
Untitled-1.gif
Then OR the 2 bytes together.
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Untitled-2.gif
This number because of the bitshift using a long, now has 16 preceding 0's.
If you OR
33023
00000000000000001000000011111111
with
FFFF0000
11111111111111110000000000000000
you get....
11111111111111111000000011111111
Which is the Unsigned Long value of 4294934783.
Which is the Signed Long value of -32512. Which is a safe integer value that doesn't cause an overflow.
Yeh now that i look at it, its extremely pointless.